제공 : 고려대학교 박창현
2-Amino-2-methyl-1,3-propanediol(Ammediol) Buffer
Stock Solutions
A: 0.2 M solution of 2-Amino-2-methyl-1,3-propanediol(21.03 g in 1000 ml of distilled water)
B: 0.2 M HCI
50 ml of A plus x ml of B and diluted to a total of 200 ml with distilled water:
x pH x pH
2.0 10.0 22.0 8.8
3.7 9.8 29.5 8.6
5.7 9.6 34.0 8.4
8.5 9.4 37.7 8.2
12.5 9.2 41.0 8.0
16.7 9.0 43.5 7.8
Acetate Buffer
Stock Solutions
A: 0.2 M solution of acetic acid (11.55 ml in 100 ml of distilled water)
B: 0.2 M solution of sodium acetate (16.4 g of C2H3O2Na or 27.2 g of C2H3O2Na·3H2O in 1000 ml of distilled water)
x ml of A plus y ml of B and diluted to a total of 100 ml with distilled water:
x y pH
46.3 3.7 3.6
41.0 9.0 4.0
30.5 19.5 4.4
20.0 30.0 4.8
14.8 35.2 5.0
10.5 39.5 5.2
4.8 45.2 5.6
Barbital Buffer
Stock Solutions
A: 0.2 M solution of sodium barbital (Veronal) (41.2 g in 100 ml of distilled water) B: 0.2 M HCI
50 ml of A plus x ml of B and diluted to a total of 200 ml with distilled water:
x pH x pH
1.5 9.2 22.5 7.8
2.5 9.0 27.5 7.6
4.0 8.8 32.5 7.4
6.O 8.6 39.0 7.2
9.0 8.4 43.0 7.0
12.7 8.2 45.0 6.8
17.5 8.0
Cacodylate Buffer
Stock Solutions
A: 0.2 M solution of sodium cacodylate 〔42.8 g of Na(CH3)2 AsO2˙3H2O in 1000 ml of distilled water〕
B: 0.2 M HCI
50 ml of A plus x ml of B and diluted to a total of 200 ml with distilled water:
x pH x pH
2.7 7.4 29.6 6.0
4.2 7.2 34.8 5.8
6.3 7.0 39.2 5.6
9.3 6.8 43.0 5.4
13.3 6.6 45.0 5.2
18.3 6.4 47.0 5.0
23.8 6.2
Citrate Buffer
Stock Solutions
A: 0.1 M solution of citric acid (21.01 g in 1000 ml of distilled water)
B: 0.1 M solution of sodium citrate (29.41 g of C6H5O7Na3˙2H2O in 1000 ml of distilled water)
x ml of A plus y ml of B and diluted to a total of 100 ml with distilled water:
x y pH x y pH
46.5 3.5 3.0 23.0 27.0 4.13
43.7 6.3 3.2 20.5 29.5 5.0
40.0 10.0 3.4 18.0 32.0 5.2
37.0 13.0 3.6 16.0 34.0 5.4
35.0 15.0 3.8 13.7 36.3 5.6
33.0 17.0 4.0 11.8 38.2 5.8
31.5 18.5 4.2 9.5 41.5 6.0
28.0 22.0 4.4 7.2 42.8 6.2
25.5 24.5 4.6
Collidine Buffer (0.2 M)
Stock Solution
s-Collidine (pure) 2.67 ml
Distilled water to make 50.0 ml
Buffer
Stock solution 50.0 ml
I N HCI 9.0 ml (approx. for pH 7.4)
Distilled water to make 100 ml
Maleate Buffer
Stock Solutions
A: 0.2 M solution of acid sodium maleate (8 g of NaOH plus 23.2 g of maleic acid or 19.6 g of maleic anhydride in 1000 ml of distilled water)
B: 0.2 M NaOH
50 ml of A plus x ml of B and diluted to a total of 200 ml with distilled water:
x pH x pH
7.2 5.2 33.0 6.2
10.5 5.4 38.0 6.4
15.3 5.6 41.6 6.6
20.3 5.8 44.4 6.8
26.9 6.0
Phosphate Buffer(S�rensen)
Stock Solutions
A: 0.2 M solution of monobasic sodium phosphate (27.8 g in 1000 ml of distilled water)
B: 0.2 M solution of dibasic sodium phosphate (53.65 g of Na2HPO4˙7H2O or 71.7 g of Na2 HPO4˙12H2O in 1000 ml of distilled water)
x ml of A plus y ml of B and diluted to a total of 200 ml with distilled water:
x y pH x y pH
93.5 6.5 5.7 45.0 55.0 6.9
92.0 8.0 5.8 39.0 61.0 7.0
90.0 10.0 5.9 33.0 67.0 7.1
87.7 12.3 6.0 28.0 72.0 7.2
85.0 15.0 6.1 23.0 77.0 7 3
81.5 18.5 6.2 19.0 81.0 7.4
77.5 22.5 6.3 16.0 84.0 7.5
73.5 26.5 6.4 13.0 87.0 7.6
68.5 31.5 6.5 10.5 90.5 7.7
62.5 37.5 6.6 8.5 91.5 7.8
56,5 43.5 6.7 7.0 93.0 7.9
51.0 49.O 6.8 5.3 94.7 8.0
Phosphate Buffer (Millonig, 1961)
Stock Solutions
A: 2.26% NaH2PO4˙H2O in water
B: 2.52% NaOH in water
Buffer (0.13 M)
Solution A 41.5 ml
Solution B 8.5 ml
The pH is 7.3 the disired pH can be obtained with solution B without changing the molarity. The buffer is stable for
several weeks at 4oC.
Tris(hydroxymethyl)aminomethane Buffer
Stock Solutions
A: 0.2 M solution of tris(hydroxymethyl)aminomethane(24.2 g in 1000 ml of distilled water)
B: 0.2 M HCL
50 ml of A plus x ml of B and diluted to a total of 200 ml with distilled water:
x pH x pH
5.0 9.0 26.8 8.O
12.2 8.6 38.4 7.6
8.1 8.8 32.5 7.8
16.5 8.4 41.4 7.4
21.9 8.2 44.2 7.2
Tris(hydroxymethyl)aminomethane Maleate Buffer
Stock Solutions
A: 0.2 M solution of Tris acid maleate 〔24.2 g of tris(hydroxymethyl)aminomethane plus 23.2 g of maleic acid or 19.6 g of maleic anhydride in 1000 ml of distilled water〕
B: 0.2 M NaOH
50 ml of A plus x ml of B and diluted to a total of 200 ml with distilled water
x pH x pH
7.0 5.2 48.0 7.0
10.8 5.4 51.0 7.2
I5.5 5.6 54.0 7.4
20.5 5.8 58.0 7.6
26.0 6.0 63.5 7.8
31.5 6.2 69.0 8.0
37.0 6.4 75.0 8.2
42.5 6.6 81.0 8.4
45.0 6.8 86.5 8.6
Veronal Acetate Buffer (Zetterqvist, 1956)
Stock Solution
Sodium veronal (barbitone sodium) 2.94 g
Sodium acetate (hydrated) ].94 g
distilied water to make 100 ml
Ringer's Solution
Sodium chloride 8.05 g
Potassium chloride 0.42 g
Calcium chloride 0.18 g
Distilled water to make 100 mI
Buffer
Stock solution 10.0 ml
Ringer's solution 3.4 ml
Distilled water 25.0 ml
0.1 M HCl 11.0 ml(approx.)